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Synthesis and characterization of the Zn-Gel. (A) Schematic representation of gel formation. (B) Digital images of the sol–gel transition and (C) hydrogel injection. (D) Phase transition time depending on ZnO 2 concentration and addition of H 2 O 2 . Rheological analysis depending on ZnO 2 concentration (E) without H 2 O 2 solution and (F) with H 2 O 2 solution. (G) Porous structure and atomic % of the hydrogels using scanning electron microscopy coupled with energy-dispersive <t>spectroscopy</t> <t>(SEM–EDS)</t> analysis. In vitro hydrogel degradation ratio using (H) collagenase type II and (I) Dulbecco’s phosphate-buffered saline (DPBS). The results in (E), (F), (H), and (I) are shown as average value ± SD ( n = 3). * indicates significant difference from Z0 (* P < 0.05; ** P < 0.01; *** P < 0.001). ## indicates significant difference from Z0.125 ( ## P < 0.01). Scale bars represent 250 μm.
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Synthesis and characterization of the Zn-Gel. (A) Schematic representation of gel formation. (B) Digital images of the sol–gel transition and (C) hydrogel injection. (D) Phase transition time depending on ZnO 2 concentration and addition of H 2 O 2 . Rheological analysis depending on ZnO 2 concentration (E) without H 2 O 2 solution and (F) with H 2 O 2 solution. (G) Porous structure and atomic % of the hydrogels using scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM–EDS) analysis. In vitro hydrogel degradation ratio using (H) collagenase type II and (I) Dulbecco’s phosphate-buffered saline (DPBS). The results in (E), (F), (H), and (I) are shown as average value ± SD ( n = 3). * indicates significant difference from Z0 (* P < 0.05; ** P < 0.01; *** P < 0.001). ## indicates significant difference from Z0.125 ( ## P < 0.01). Scale bars represent 250 μm.

Journal: Biomaterials Research

Article Title: Zinc Peroxide-Mediated In Situ Forming Hydrogels for Endogenous Tissue Regeneration

doi: 10.34133/bmr.0238

Figure Lengend Snippet: Synthesis and characterization of the Zn-Gel. (A) Schematic representation of gel formation. (B) Digital images of the sol–gel transition and (C) hydrogel injection. (D) Phase transition time depending on ZnO 2 concentration and addition of H 2 O 2 . Rheological analysis depending on ZnO 2 concentration (E) without H 2 O 2 solution and (F) with H 2 O 2 solution. (G) Porous structure and atomic % of the hydrogels using scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM–EDS) analysis. In vitro hydrogel degradation ratio using (H) collagenase type II and (I) Dulbecco’s phosphate-buffered saline (DPBS). The results in (E), (F), (H), and (I) are shown as average value ± SD ( n = 3). * indicates significant difference from Z0 (* P < 0.05; ** P < 0.01; *** P < 0.001). ## indicates significant difference from Z0.125 ( ## P < 0.01). Scale bars represent 250 μm.

Article Snippet: To analyze the surface morphology and elemental composition of Zn-Gel, scanning electron microscopy (SEM) coupled with energy-dispersive spectroscopy (EDS) analysis (SEM–EDS) was performed using a JSM 7001F microscope (JEOL, Tokyo, Japan); 200-μl hydrogel samples with varying ZnO 2 concentrations (ranging from 0 to 0.5 wt%) were prepared within 1-ml syringes for this analysis.

Techniques: Injection, Sublimation, Concentration Assay, Electron Microscopy, Spectroscopy, In Vitro, Saline